40818 DATASHEET PDF

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Incubate membrane in 25 datasueet of blocking buffer for 1 hr at room temperature. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser of human STING protein. Adjust pH to 8. Appropriate isotype controls are highly recommended in order to show specific binding in your primary antibody immunoprecipitation. Would you like to visit your country specific website?

From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Incubate 30 min on ice. Proceed with detection Section D. Wash cells by centrifugation in excess 1X PBS to remove methanol. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro of human USP8 protein.

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Electrotransfer to nitrocellulose membrane Treat cells by adding fresh media containing regulator for desired time. Count cells using a hemocytometer or alternative method. Primary Antibody Dilution Buffer: Discard supernatant in appropriate waste container. Datasbeet of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. Incubate for at least 5 min at 408818 temperature.

Place tube back in magnetic separation rack.

CST – Phospho-STING (Ser) (D8K6H) Rabbit mAb

Aspirate fixative, rinse three times in 1X PBS for 5 min each. Allow cells to fix for 15 min at room temperature. Recommended Anti-Rabbit secondary antibodies: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution. Immunoprecipitation for Native Proteins This protocol is intended for immunoprecipitation of native proteins for analysis by western immunoblot or kinase activity utilizing Protein A magnetic separation.

Proceed to immunoprecipitation section. Incubate with rotation for 20 minutes at room temperature. Analyze sample by western blot see Western Immunoblotting Protocol.

Find answers on our FAQs page. Resuspend cells in 0.

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Blotting Membrane and Paper: Place the tube in a magnetic separation rack for seconds. Incubate specimen in fluorochrome-conjugated secondary antibody diluted in Antibody Dilution Buffer for 1—2 hr at room temperature in the dark. Remove PBS and add 0. Western blot analysis of extracts from various cell lines using USP8 Antibody. Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Solutions and Reagents Achieve higher quality immunofluorescent images using the efficient and cost-effective, pre-made reagents in our Immunofluorescence Application Solutions Kit NOTE: Aliquot desired number of cells into tubes or wells.

Pre-clear enough lysate for test samples and isotype controls. Application Dilutions Western Blotting 1: The supernatant is the cell lysate.

Phospho-STING (Ser366) (D8K6H) Rabbit mAb #40818

Formaldehyde is toxic, use only in a fume hood. Prepare solutions with reverse dtaasheet deionized RODI or equivalently purified water.

Pellet beads using magnetic separation rack. USP8 Antibody – Carefully remove the buffer once the solution is clear.

Cells should be grown, treated, fixed and stained directly in multi-well plates, chamber slides or on coverslips.